Analysis Note

ControlMouse ovary tissue lysate

Application

Research CategoryCell Structure

Detect TNF-alpha induced protein 6 (TSG-6) using this Anti-TNF alpha Antibody, clone NG3 validated for use in WB, ELISA. This TNF alpha antibody has been tested in Human and Mouse.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tumor Necrosis Factor-alpha (TNF-alpha), also known as TSG-6, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. TNF-alpha induced protein 6 may be involved in cell-cell and cell-matrix interactions during normal inflammatory responses and during tumorigenesis.

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to mouse TSG-6.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse ovary tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TSG-6 in 10 µg of mouse ovary tissue lysate.

Specificity

Published to react with human TSG-6 (Nagyeri, G., et al. (2011). JBC. PMID: 21566135).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~29 kDa observed.
An uncharacterized band appears at ~50 kDa in some lysates.